Study on absorption and metabolism of active compounds of polygoni multiflori radix in hepatic L-02 cells by liquid chromatography tandem-mass spectrometry
- Author:
Pei LIN
1
Author Information
- Publication Type:Journal Article
- Keywords: Absorption; Emodin; LC-MS/MS; Metabolism; Stilbene glucoside
- From: Chinese Pharmaceutical Journal 2015;50(12):1048-1053
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To study the absorption and metabolism of active compounds of Polygoni Multiflori Radix in L-02 cells and elucidate their chemical structures by liquid chromatography tandem-mass spectrometry. METHODS: The active compounds in Polygoni Multiflori Radix, 2,3,5,4'-tetrahydroxystilbene-2-O-γ-5-glucoside (TSG) and emodin (EN), were cultured into L-02 cells. Cell culture media and cell lysis were collected after 0,2,4,6,8,12,24,36 and 48 h respectively. Reversed-phase high-performance liquid chromatography (HPLC) was used to explore the possible absorption and metabolism. Liquid chromatography-tandem-mass spectrometry (LC-MS/MS) was used to identify the chemical structures of the metabolic products. RESULTS: TSG showed no obvious accumulation in L-02 cells. One metabolic product of TSG was detected by HPLC, and its concentration increased with lime after 8 h. This metabolite was identified as the glucuronidation of TSG by LC-MS/MS. In contrast, EN was partially accumulated in L-02 cells. Three isomers of glucuronidations and one sulfation of EN were also identified by LC-MS/MS. CONCLUSION: EN is easier to be absorbed and accumulated in L-02 cells than TSG. Phase II metabolic reactions play an important role in the metabolism of TSG and EN in cells. However, the accurate binding sites need to be further confirmed.
