Protective effect of palmitic acid against glutamate-induced apoptosis in PC12 cells
- Author:
Jing LI
1
Author Information
- Publication Type:Journal Article
- Keywords: Apoptosis; Glutamic acid; Palmitic acid; PC12 cells; Protection
- From: Chinese Pharmaceutical Journal 2015;50(13):1117-1122
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To explore the protective effect of palmitic acid against glutamate-induced apoptosis in PC12 cells. METHODS: PC12 cells were randomly divided into six groups: normal group, solvent group(DMSO 0.1%), model group (Glu 25 mmol · L-1), nimodipine(NMP, 7 μmol · L-1)-treated group or MK801 (10 μmol · L-1) -treated group set as positive control group, palmitic acid groups of 100, 10, 1 μmol · L-1 dose. Cell viability and membrane permeability were examined by MTT assay and LDH kit. Morphologic change of apoptosis were detected by AO/EB and Hoechst staining with fluorescence microscope. The apoptosis rates were measured by flow cytometry using fluorescein labeling FITC-Annexin V/PI. The expression of Bcl-2, Bax and GluR1 protein was assayed by Western blot methods. RESULTS: Compared with the model group, the results showed that palmitic acid could significantly improve the viability of PC12 cells damaged by glutamate, and reduce the permeability of cell membranes. After AO/EB staining, nuclear chromatin of the cells in model group were stained strongly into orange by EB. In palmitic acid - treated group, only few cells were stained into orange. Compared with model group, after the treatment of palmitic acid, slight blue fluroscence stained by Hoechst in nucleus had been emitted. Palmitic acid could increase the expression of Bcl-2 and inhibit the level of the expression of Bax protein and GluR1 protein. CONCLUSION: In summary, palmitic acid had protective effect on glutamate-induced apoptosis in PC12 cells through increasing the expression of Bcl-2 and inhibiting the expression of Bax and GluR1.
