Analysis of difference in chemical constituents of Radix Pseudostellariae from different germplasms by UPLC-Triple TOF-MS/MS
- Author:
Ya HOU
1
Author Information
- Publication Type:Journal Article
- Keywords: Chemical composition; Germplasm resource; Radix Pseudostellariae; UPLC-triple TOF-MS/MS
- From: Chinese Pharmaceutical Journal 2015;50(13):1104-1110
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To analyze the differences in chemical composition of Pseudostellariae Radix from different germplasms. METHODS: Tweny-four batches of samples were determined by UPLC-Triple TOF-MS/MS. Characteristic peaks were extracted through treatment of mass spectrometry data including peak matching, peak alignment and noise filtering. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used for data processing. Component identification was carried out based on the mass spectrometry accurate mass and secondary mass spectrometry fragmentation information combined with database search and literature. RESULTS: The differences among samples of Pseudostellariae Radix of different germplasms were distinguishable. Twenty-one chemical compositions with significant differences were screened, among which 10 common chemical compositions showed different changing laws. CONCLUSION: This study provides experimental data for revealing the laws of Pseudostellariae Radix metabolites from different germplasms.
