Effect of mangiferin on superoxide dismutase isozyme expression in rats with chronic bronchitis
- Author:
Zhi-Quan WEI
1
Author Information
- Publication Type:Journal Article
- Keywords: Bronchial epithelial cells; Chronic bronchitis; Isozyme; Mangiferin; Superoxide dismutase
- From: Chinese Pharmaceutical Journal 2015;50(11):941-946
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: This research was designed to investigate the regulation of mangiferin on superoxide dismutase (SOD) isozyme expressionin rats with cigarettesmoke-induced chronic bronchitis, plus withthe protection on bronchial epithelial cellsultrastructure and anti-inflammatory action. METHODS: The rat model with chronic bronchitis was established by cigarette smoke. Real-time fluorescence RT-PCR was executed for evaluating the SOD1, SOD2 and SOD3 gene expression in lung tissue, and enzyme-linked im-muno sorbent assay (ELISA) for SOD1, SOD2 and SOD3 protein level. As well, interleukin-1 (IL-1), tumor necrosis factor-a (TNF-a) and malondialdehyde (MDA) level in lung tissue were detected by ELISA. Furthermore, the bronchial epithelial cellsultrastructure was observed under transmission electron microscopy. The histopathological images was obtained by lung tissue HE staining slides. RESULTS: The cigarette smokeresulted in a markedly down-regulation expressions of SOD1, SOD2 and SOD3 in lung tissue. The down-regulation expressions of SOD1 and SOD2 in lung tissue cannot be antagonized by mangiferin. However, mangiferin significantly inhibited the down-regulation of SOD3, and markedly decreased IL-1, TNF-a and MDA. Furthermore, the structural completeness of the bronchial epithelial cellsultrastructure was maintainedin a good attitude by mangiferin, while the greatly reduced chronic bronchitis was found. CONCLUSION: Mangiferin can obviously reduce the chronic bronchitis induced by cigarette smoke and keep the bronchial epithelial cells from damage. The protective action might not rely only on anantagonistic action on down-regulated SOD3 expression in lung tissue by mangiferin.
