Metabolism rule of secondary metabolites, steroidal saponins active ingredients, in Paris polyphylla var. yunnanensis Franch.
- Author:
Li-Li FENG
1
Author Information
- Publication Type:Journal Article
- Keywords: Paris polyphyUa var. yunnanensis Franch.; Secondary metabolite; Ultra performance liquid chromatography
- From: Chinese Pharmaceutical Journal 2015;50(8):664-670
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To discuss the metabolism rule of the active steroidal saponins in the roots, stems and leaves of Paris polyphylla var. yunnanensis Franch belonging to different populations and different plants in the same population. METHODS: UPLC was used to determine the contents of the active ingredients including polyphyllin I, polyphyllin II, polyphyllin VI, and polyphyllin VII from the roots, stems, and leaves, the UPLC map was established, the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine software (Version 2004 A) developed by the Chinese Pharmacopoeia Committee was used to analyze the number of peaks in the UPLC map, and One-way ANOVA was used to analyze their metabolism rule. RESULTS: There was significant difference in the contents of polyphyllin I, polyphyllin II, polyphyllin VI, polyphyllin VII, and their total contents in the roots, stems, and leaves among different populations (P < 0.01). The number of peaks in the UPLC map ranked in the folloing order: leaf > root > stem. The difference among different plants of the same Paris polyphyUa var. yunnanensis Franch population was smaller. No. 1, 2, 4, 5 peaks all existed in the UPLC maps of the roots, stems, and leaves, but there were 11 and 16 more peaks in the leaves than the roots and stems, respectively. CONCLUSION: There are fewer kinds of main active ingredients with low contents in the stems of Paris polyphyUa var. yunnanensis Franch. The population genetic capabilities of the metabolism of the steroidal saponins active ingredients are stable in the roots, stems and leaves of different plants from the same population, but there are large differences between different populations. Further pharmacological, toxicological, and clinical studies need to be done to determine whether the leaves and stems could lake the place of root as an alternative medicine.
