Simultaneous determination of seven phenolic acids from Xanthii Herba and Xanthii Fructus by HPCE
- Author:
Juan-Xiu LIU
1
Author Information
- Publication Type:Journal Article
- Keywords: Cynarin; Determination; HPCE; Phenolic acid; Xanthii Fructus; Xanthii Herba
- From: Chinese Pharmaceutical Journal 2015;50(4):366-370
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish a high performance capillary electrophoresis (HPCE) method for simultaneousn determination of nchlorogenic acid, caffeic acid, ferulic acid, isochlorogenic acid A, isochlorogenic acid C, neochlorogenic acid, and cynarin in the herbs of Xanthii Herba and Xanthii Fructus. METHODS: 50 mmol·L-1 borax-water was used as buffer solution (pH 9.54). The separation was performed on an uncoated fused silica capillary (64.5 cm×75 μm, 56 cm of effective length) maintained at 25℃ at voltage of 25 kV. The detection wavelength was 326 nm, and the sample was injected at 25 kPa×6 s. RESULTS: The calibration curves of the seven phenolic acid showed good linearity (r>0.9994) in the ranges of the tested concentrations, and the average recoveries of the method were between 99.85%-102.70%. CONCLUSION: The method is simple, accurate and reproducible, and can be used for the quality evaluation and control of Xanthii Herba and Xanthii Fructus.
