Comparative study on the total flavonoids content in different species of bupleurum in different growth period on the site
- Author:
Ying-Zi DUAN
1
Author Information
- Publication Type:Journal Article
- Keywords: Bupleurum chinense; Flower; Total flavonoids; UV-visible spectropholometry
- From: Chinese Pharmaceutical Journal 2015;50(5):394-398
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To study variation of total flavonoids from nine different Bupleurum chinense introduced of different ground parts of different organs in different growth period, and explore the best harvest period and the optimal varieties. METHODS: The extraction conditions were optimized by microwave assisted method, UV-visible spectropholometry was used to determine the contents of total flavonoids. RESULTS: The optimum extraction conditions: the concentration of ethanol was 80%, each microwave time 40 s, microwave 10 times; the content of total flavonoids was difference in different growth period, fluctuated trend. The flower was the highest, with an average of 7.59% in the range of 4.65%-16.75%, the content of total flavonoids in nutrition growth phase; leaf>stem, in flowering stage; flower>leaf>stem; in fruiting stage; the content of Gansu Longxi, Liaoning Shenyang, Shandong Heze and Hebei Anguo Bupleurum: fruit>leaf>stem, other Bupleurum; leaf>stem>fruit; The higher the content of total flavonoids was in the flowering and fruiting of nine different Bupleurum chinense introduced, Gansu Longxi Bupleurum (flowering stage) was the highest, up to 27.41%. CONCLUSION: It is suggested that harvested in the flowering or fruiting stage, and carried on the comprehensive development and utilization.
