In vivo evaluation of transdermal delivery properties of evodiamine and rutacarpine by skin microdialysis and HPLC method
- Author:
De-Tang LI
1
Author Information
- Publication Type:Journal Article
- Keywords: Dermal in vivo microdialysis; Evodiamine; Rutacarpine
- From: Chinese Pharmaceutical Journal 2015;50(3):239-243
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To determine the concentrations of evodiamine and rutacarpine in subcutaneous tissues of rats after administration of evodiae extract to evaluate the transdermal drug release properties. METHODS: The rats were treated with abdominal hair removal, followed by covering with black polyethylene film transdermal patch of evodiae extract. The concentrations of evodiamine and rutacarpine in the subcutaneous tissues at different time points were measured by microdialysis sampling technique combined with HPLC method. RESULTS: The maximum concentration(ρ
max ) of evodiamine in the dialysate was(417.24 ± 43.44) μg · mL-1, time to peak concentration (tmax ) was (4.210 ± 0.630) h, and the area under the concentration time curve (AUC0→20 ) was (3 189.31 ± 789.97) μg · h · mL-1; the ρmax of rutacarpine was(287.13 ± 26.78) μg · mL-1, tmax was (3.980 ± 0.580) h, and the AUC0→16 was(l327.97 ± 245.87) μg · h · mL-1. CONCLUSION: In vivo microdialysis sampling technique combined with HPLC method can be used for the evaluation of transdermal drug release characteristics of evodiamine and rutacarpine. The method has the advantages of simple operation with high sensitivity and specificity. Evodiamine and rutacarpine in evodiae extract have the characteristics of slow transdermal penetration speed but high penetration amount.
