Effects of guavenoic acid on protection against oxidative damage in INS-1 Cells and its mechanisms
- Author:
Jing-Ru WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Guavenoic acid; INS-1β cells; Oxidative damage
- From: Chinese Pharmaceutical Journal 2015;50(3):233-238
- CountryChina
- Language:Chinese
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Abstract:
OBJECTIVE: To investigate the effects of guavenoic acid(GA) on protection against oxidative damage in INS-1 cells. METHODS: INS-1β cells were cultured in vitro. Control group, medium group, model group, drug groups and positive group were set. INS-1 cells were treated with GA in 0.3, 1, 3, 10, 30 nmol · L-1 for 48 h. Using 650 μmol · L-1 H
2 O2 challenges the INS-1 cells for 2 h to establish the oxidative damage model(OD), the cell viability was tested by MTT assay, the nuclear was stained by Hoechst 33258, the ROS in OD-INS-1 was tested by ROS-kit and the apoptosis was tested via double staining-fcm. RESULTS: Compared with model group, at low concentration GA(0.3, 1 nmol · L-1) could protect INS-1 cells exposed to H2 O2 significantly (P < 0.05 or P < 0.01). Nuclear staining results showed that compared with the oxidative damage model group, cells treated with 0.3 nmol · L-1 GA, and emitting weaker cell fluorescence. 0.3, 1, 3 nmol · L-1 GA could reduce the DCF fluorescence intensity very significantly (P < 0.01). At 0.3 nmol · L-1, GA can significantly resist the occurrence of apoptosis(P < 0.05). CONCLUSION: GA could protect INS-1 cells from oxidative damage, maybe achieved by inhibiting intracellular ROS generation and against apoptosis.
