Determination of ASC-J9 in rat blood by HPLC-MS/MS
- Author:
Feng-Fei WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Blood concentration; HPLC-MS/MS; KASC-J9; Pharmacokinetics
- From: Chinese Pharmaceutical Journal 2015;50(22):1996-1999
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish an HPLC-MS/MS method for determination of ASC-J9 in rat blood. METHODS: After liquid-liquid extraction, ASC-J9 was separated on a Symmetry Cl8 colume, with mobile phase of acetonitrile-water containing 0.1% formic acid and 10 mmol·L-1 ammonium for mate. The flow rate was 1.0 mL·min-1, mass shunt was 0.4 mL·min-1, and column temperature was main tained at 35℃. Quantification was performed in positive ion multiple-reaction-monitoring (MRM) mode. RESULTS: The calibration curve of ASC-J9 had good linearity in the concentration range of 3.54-1180 ng·mL-1. The extraction recovery rate was within 83.19% to 87.27%, and the intra-day and inter-day RSDs were both less than 8.89%. CONCLUSION: This method is specific, sensitive and suitable for determination of ASC-J9 in rat blood.
