In vitro anticancer activity of doxorubicin-loaded heparinized magnetic mesoporous silica nanoparticles
- Author:
Hai-Yan WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Doxorubicin; Heparin; HepG2 cell; Mesoporous silica
- From: Chinese Pharmaceutical Journal 2015;50(24):2130-2134
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To prepare doxorubicin-loased heparinized magnetic mesoporous silica nanoparticles (MMSNs-HP) and investigate their drug release profile and anticancer activity in vitro. METHODS: Amino-modified MMSNs was synthesized by combining phase transfer method with sol-gel method firstly. Then heparin was conjugated with the above nanoparticles via carbodiimide chemistry to form MMSNs-HP. Finally, the following experiments were performed, such as loading/release of doxorubicin into/from MMSNs-HP in vitro, cellular uptake of MMSNs-HP by hepatoma cell HepG2 and cell cytotoxicity of doxorubicin-loaded MMSNs-HP. RESULTS: MMSNs-HP was able to delay the release of doxorubicin significantly, penetrate into tumor cells, kill HepG2 cell, and inhibit the proliferation of HepG2 cells induced by basic fibroblast HepG2 cells (bFGF). CONCLUSION: MMSNs-HP is a potential drug carrier for the delivery of antitumor drugs.
