Optimization of preparation of lignum dalbergia odorifera oil-hydroxyl-β-cyclodextrin inclusion complex by central composite design-response surface method
- Author:
Xi LI
1
Author Information
- Publication Type:Journal Article
- Keywords: Central composite design-response surface method (CCD-RSM); Hydroxypropyl-β-cyclodextrin; Inclusion complex; Lignum dalbergia odorifera oil; Preparation process
- From: Chinese Pharmaceutical Journal 2016;51(2):120-126
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To prepare the inclusion complex of Lignum dalbergia odorifera oil with hydroxyl-β-cyclodextrin (HP-β-CD), and to optimize the preparation process of it. METHODS: The inclusion complex was prepared by the stirring-freeze-dry meth od. The preparation process was optimized by central composite design-response surface method (CCD-RSM), with the colligation score which was calculated by the yield of inclusion, the utilization rate of volatile oil and the content of trans-nerolidol as index. The inclu sion complex was verified by phase-solubility method, DSC,UV and microscopical identification. RESULTS: The optimum inclusion technology was: inclusion solvent 5% ethanol, stirring rate 500 r·min-1, HP-β-CD to volatile oil 33:1, inclusion temperature 42℃, inclusion time 2.5 h. The formation of inclusion complex can change the solubility, optical and thermodynamic properties of volatile oil. CONCLUSION: The preparation process of inclusion complex of Lignum dalbergia odorifera oil with HP-β-CD optimized by CCD-RSM is reasonable and feasible, and provide a reliable experiment basis for its application.
