Mechanism of ethyl gallate induced the apoptosis of Lovo cells through mitochodrial pathway
- Author:
Bai-Yu JIAN
1
Author Information
- Publication Type:Journal Article
- Keywords: Apoptosis; Ethyl gallate; Human colon carcinoma Lovo cells; Mitochodrial pathway
- From: Chinese Pharmaceutical Journal 2016;51(6):463-467
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To study the effect of ethyl gallate in inducing apoptosis of human colon carcinoma Lovo cells. METHODS: Cultured human colon carcinoma Lovo cells in vitro were used as the research object. Morphological changes of apoptotic cells were observed by inverted microscope.AnnexinV-FITC/PI assay and DNA Ladder assay were performed to study the apoptotic effects. Immunocytochemistry was used to detect the expression of cleaved caspase-9 and cleaved caspase-3 proteins. RESULTS: Ethyl gallate had growth inhibition on colon cancer Lovo cells,the density was decrease: the apoptotic rates were increased with dose increase: Agarose gel electrophoresis showed evident DNA fragmentation: the expression of cleaved caspase-9 and cleaved caspase-3 proteins was increased in treated groups. CONCLUSION: Ethyl gallate could induce the apoptosis in Lovo cells through up-regulating the expression of cleaved caspase-9 and cleaved caspase-3 protein,and then activated the mitochondrial pathway.
