Sustained-release effect of Na-montmorillonite on 1-deoxynojirimycin in alkaloid fraction of mulberry leaf extract
- Author:
Chao DENG
1
Author Information
- Publication Type:Journal Article
- Keywords: DNJ; HPLC; Montmorlillonite; Mulberry leaf extract; Sustained-release preparation
- From: Chinese Pharmaceutical Journal 2016;51(8):639-644
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To investigate the sustained-release ability of Na-montmorillonite (Na-MMT) on 1-deoxynojirimycin (DNJ) in mulberry leaf extract (MLE). METHODS: In order to prepare DNJ-MMT composite, DNJ was intercalated into the inter-layer of MMT by stirring. Time and temperature for adsorption and ratio of DNJ to MMT were determined by calculating the loading of DNJ, then in vitro release experiment was carried out to estimate the sustained-release characteristics of DNJ-MMT. RESULTS: DNJ-MMT composite with a drug loading of 111.64 mg·g-1 was obtained by stirring 5.00 g Na-MMT and 16.00 g MLE containing 4016.0 mg DNJ in 1000 mL deionized water at 100℃ for 1 h. The composite released DNJ quickly in water, potassium phosphate buffer (pH 7.4), and 0.1 mol·L-1 hydrochloric acid solution. With the increasing of volume of hydrochloric acid solution, the release ratio of DNJ also increased correspondingly. CONCLUSION: The sustained-release effect of Na-MMT on DNJ is proved. Na-MMT can be used for study of DNJ sustained-release preparations.
