Optimization of extration process of protein and polysaccharides from Antler Velvet assisted by high intensity pulsed electric fields
- Author:
Si-Min DONG
1
Author Information
- Publication Type:Journal Article
- Keywords: Antler Velvet; High intensity pulsed electric field; Polysaccharide; Process optimization; Protein
- From: Chinese Pharmaceutical Journal 2016;51(8):620-624
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To optimize the extraction proces of protein and polysaccharides from Antler Velvet using high intensity pulsed electric fields. METHODS: With water as solvent, electric field intensity, pulse number and solid-liquid ratio as investigation parameters, an L9(34) orthogonal test was used to determine the optimal process for simultaneous extraction of protein and polysaccharides from Antler Velvet. RESULTS: When the extraction by high intensity pulsed electric fields was conducted at the solid-liquid ratio of 1:30 g·mL-1 and electric field intensity of 20 kV·cm-1, with pulse number of 10, the protein extraction rate reached 12.32%, and polysaccharides extraction rate could reach 3.25%. CONCLUSION: The optimized process can effectively and simu-taneously extract protein and polysaccharides from Antler Velvet.
