Simultaneous determination of ginsenoside Rg1, Re, Rb1 and Jujuboside A, B in Renshen Jianpi pellets by HPLC
- Author:
Hong-Yan ZHU
1
Author Information
- Publication Type:Journal Article
- Keywords: Ginsenoside Rb1; Ginsenoside Re; Ginsenoside Rg1; HPLC; Jujuboside A; Jujuboside B; Renshen Jianpi Pellets
- From: Chinese Pharmaceutical Journal 2016;51(9):751-756
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish an HPLC method for simultaneous determination of ginsenoside Rg1, Re, Rb1 and jujuboside A, B in Renshen Jianpi Pellets (RSJPW). METHODS: The determination was conducted on an Eclipse XDB C18 column (4.6 mm × 250 mm, 5 μm) with the mobile phase of acetonitrile (A)-water (B) gradiently eluted at the following flow rates: 0-90 min, 1.0 mL · min-1; 90-130 min, 1.0-0.5 mL · min-1; 130-140 min, 0.5 mL · min-1. And the column temperature was maintained at 35℃; the detection wavelength was set at 203 nm. RESULTS: The calibration curves of ginsenoside Rg,, Re, Rb, and jujuboside A, B were in good linearity over 0.32-2.24 μg (r=0.9982), 0.16-1.12 μg (r=0.995 3), 0.32-2.24 μg (r=0.9996), 0.16-1.12 μg (r=0.9915), 0.08-0.56 μg (r=0.9996). The corresponding average recovery rates were 97.18%, 97.62%, 98.79%, 98.48%, 94.51%; the standard deviations were 1.10%, 0.98%, 0.34%, 1.09%, 1.88%, respectively. CONCLUSION: The established HPLC method is accurate, reliable and reproducible, which can be used as a reference for the quality control of RSJPW.
