Icariin ameliorates LPS/TLR4 induced hepatic stellate cell activatio and autophagy via FXR
- Author:
Ru-Hui YANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Autophagy; Hepatic stellate cells; Icariin; Toll-like receptor 4
- From: Chinese Pharmaceutical Journal 2016;51(9):708-714
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To investigate the effects of icariin (ICA) on autophagy and activation of HSC-T6 cell line induced by lipopolysaccharide (LPS), and further research whether this effect is associated with FXR protein. METHODS: The HSC-T6 hepatic stellate cell was stimulated with LPS and then treated with ICA of three different doses and TLR4 siRNA. Autophagy changes were observed through the acridine orange (AO) and MDC staining, expression of Beclin-1 and LC3B were determined via laser scanning confocal microscopy, cell mitochondrial membrane potentials were detected using flow cytometry and the proteins of NF-κB (p65), LC3B-II, CollagenI, TGF-(β1, α-SMA were measured through Western blot. Furthermore, the ICA treatment group was intervened with Z-Gug-gulsterone(Z-Gug). The expressions of LC3B, Beclin-1 and FXR were measured through Western blot. RESULTS: Compared with the normal group, LPS significantly increased autophagy, up-regulated expression of Beclin-1 and LC3B, decreased mitochondrial membrane protentials and increased expression of NF-κB(p65), LC3B-II, Collagen I, TGF-β1 and α-SMA. However, the effects of LPS on HSC-T6 could be partly inhibited by TLR4 siRNA and ICA in a dose-dependent manner. Moreover, ICA increased expression of FXR and could be blocked by Z-Gug treatment. CONCLUSION: ICA ameliorated LPS/TLR4 can induce hepatic stellate cell activation by reduction autophagy and association with FXR protein.
