The quality evaluation of aerial roots of ficus microcarpa L.f. based on their anti-inflammation activity
- Author:
Kun JIANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Anti-inflammation; Ficus microcarpa aerial root; HPLC; Quality evaluation; Spectrum-effect relationship
- From: Chinese Pharmaceutical Journal 2016;51(12):1033-1038
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish the quality evaluation method of the aerial roots of Ficus microcarpa L.f. based on their anti-inflammation activity. METHODS: Twenty batches of samples from different producing areas were analyzed by HPLC on a Shimadzu Capcell Pak C18 column(4.6 mm×250 mm,5 μm) gradiently eluted with mobile phase of methanol and 0.1% formic acid aqueous at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 254 nm, and the column temperature was maintained at 35℃. The chromatograms were analyzed by the software "Similarity Evaluation System for Chromatographic Fingerprint of TCMs (Version 2012.1)" and the common peaks were obtained. The anti-inflammatory effects of the aerial roots of Ficus microcarpa L.f. were assessed by murine model of xylene-induced ear edema. Spectrum-effect relationship was analyzed by bivariate correlation analysis using SPSS21.0. RESULTS: Seventeen common peaks were identified in the HPLC fingerprints of 20 batches of aerial roots of Ficus microcarpa L.f. The anti-inflammatory effect of samples from Guangdong was better than those from Guangxi and Fujian (P<0.01). According to the result of spectrum-effect relationship analysis, six common peaks were closely related to the anti-inflammatory effects of the aerial roots of Ficus microcarpa L.f. (P<0.05). Cluster analysis was then carried out based on the six common peaks in order to divide the samples into different groups. CONCLUSION: The quality evaluation method established in this research is successfully employed in the quality evaluation of the aerial root of Ficus microcarpa L.f..
