Antitumor effect of alcohol extract of pileostegia tomentella on H22 tumor-bearing mice

Ying Liu

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Antitumor effect of alcohol extract of pileostegia tomentella on H22 tumor-bearing mice

Author: Ying LIU ¹
Author Information

1. Guangxi Institute of Traditional Medical and Pharmaceutical Sciences, Guangxi Key Laboratory of Traditional Chinese Medicaine Quality Standands
Publication Type:Journal Article
Keywords: Alcohol extract; Antitumor; H22 tumor-bearing mice; IL-2; Pileostegia tomentella; TNF-α
From: Chinese Pharmaceutical Journal 2016;51(12):981-984
CountryChina
Language:Chinese
Abstract: OBJECTIVE: To study the antitumor effect of Pileostegia tomentella 95% alcohol extract (PTAE) on H22 tumor-bearing mice and its possible mechanisms. METHODS: Sixty mice were chosen and mouse models bearing H22 solid tumor were established in fifty mice, and the others were as normal control. H22 tumor-bearing mice were randomly divided into five groups:model control group, fluorouracil group(20 mg·kg-1), PTAE high, middle and low-dose group(180, 90, 45 g·kg-1 of crude drug, respectively). The mice in treatment groups were intragastric administration respectively, meanwhile, the mice in normal control and model groups were treated with the same volume of distilled water, once a day for ten days. The blood was collected from eyeball in all mice, and the serum were separated and detected by ELISA for IL-2 and TNF-α. Then the mice were put to death. Their tumors, thymuses and spleens were separated and weighted, and the tumor inhibitory rates, thymus and spleen indexes were calculated. The pathological change of tumor tissue was observed. RESULTS: Compared with model control group, the tumor weights of PTAE high and middle-dose groups were significantly decreased (P<0.05, P<0.01), the tumor inhibitory rates were 37.44% and 38.46% respectively. The spleen index of PTAE middle-dose group was increased significantly (P<0.01). The level of IL-2 in serum of tumor-bearing mice in the PTAE high-dose group was increased significantly(P<0.05), and the level of TNF-α in serum (P<0.01) could be increased significantly in the PATE high, middle and low-dose groups. CONCLUSION: Pileostegia tomentella 95% alcohol extract has antitumor activity, its mechanism may be developed by immuno-regulation.