Simultaneous determination of multiple constituents in scrophulariae radix from different habitats and commercial herbs by UPLC-QTRAP-MS/MS
- Author:
Sheng-Nan WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Iridoid; Organic acid; Phenylpropanoid glycoside; Scrophulariae Radix; UPLC-QTRAP-MS/MS
- From: Chinese Pharmaceutical Journal 2016;51(16):1416-1422
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To develop a method for the determination of iridoid, phenylpropanoid glycosides, and organic acids in Scrophulariae Radix from different habitats and commercial herbs by UPLC-QTRAP-MS/MS. METHODS: The analysis was carried out on a BDS HYPERSIL C18 column (4.6 mm×250 mm, 5 μm) with elution by mobile phase of acetonitrile-water at a flow rate of 1.0 mL·min-1. The column temperature was maintained at 35℃. The target compounds were analyzed by the negative ion multiple reaction monitoring (MRM) mode. RESULTS: Twelve multiple constituents showed good linearity (r>0.9994) in the range of the tested concentration. The average recoveries of the 12 components were 99.59%-101.24% with relative standard deviations of 0.93%-1.60%. CONCLUSION: The established method is accurate and precise, which provides a reliable and effective technique for the quality evaluation of Scrophulariae Radix.
