Effects of combination with SAHA and TRAIL treatment on cell growth of er positive breast cancer cell line MCF-7
- Author:
Xiu-Yan FENG
1
Author Information
- Publication Type:Journal Article
- Keywords: Cell apoptosis; Estrogen receptor positive breast cancer; Suberoylanilide hydroxamic acid (SAHA); TNF-related apoptosis inducing ligand (TRAIL)
- From: Chinese Pharmaceutical Journal 2016;51(16):1373-1378
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To study the effects of suberoylanilide hydroxamic acid (SAHA) and TRAIL treatment on cell proliferation and apoptosis for ER positive breast cancer cell line MCF-7. METHODS: Human breast cell lines (MCF-7) were evaluated for the expressions of cell viability, cell apoptosis and cell cycle by muse cell analyzer. The mRNA levels of related apoptotic factors in MCF-7 cells were detected by real time PCR and solid phase apoptosis antibody microarray. RESULTS: After the combination with SAHA and TRAIL, the ability of cell proliferation and cell viability were depressed, and the cell apoptosis was induced. The cell cycle assay showed that the MCF-7 cells were arrested in G0/G1 phase with SAHA and TRAIL treatment. CONCLUSION: The combinatorial treatment of SAHA and TRAIL has a significantly inhibitory effect on cell growths of ER positive breast cancer MCF-7 cell.
