Involvement of mitochondria-mediated pathway and Akt/GSK3β signaling in protective action of albi-florin against MPP+-induced PC12 cells apoptosis
- Author:
Xin-Yu HU
1
Author Information
- Publication Type:Journal Article
- Keywords: 1-methyl-4-phenylpyridinium; Albiflorin; Neuro-protection; PC12 cells
- From: Chinese Pharmaceutical Journal 2016;51(17):1467-1471
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To determine the neuro-protective effects of albiflorin against MPP+-induced PC12 cells death and related molecular mechanisms. METHODS: PC12 cells were treated with 4 mmol·L-1 MPP+ to establish apoptotic cell models. MTT method, DCFH-DA staining, JC-1 staining and Western Blot were used to determine the changes of cell viability, intracellular ROS concentration, mitochondrial membrane potential and the expression of Bel-2 and Bcl-X1, and the phosphoration of Akt/GSK3β in PC12 cells. RESULTS: Albiflorin showed significantly neuro-protective effects against MPP+-induced cell damage. Results showed that albiflorin enhanced the cell viability, reduced intracellular ROS level and caspase 3 activation, restored the mitochondrial membrane potential, increased the expression of Bcl-2 and Bcl-X1, and enhanced the phosphoration of Akt/GSK3β. CONCLUSION: Albiflorin shows neuro-protective effects against MPP+-induced PC12 cells damage via mitochondrial-dependent pathway and Akt/GSK3β signaling.
