Chemical constituents isolation of dendrobium officinale based on supercritical fluid extraction and simulated moving bed
- Author:
Zheng-Ming TAO
1
Author Information
- Publication Type:Journal Article
- Keywords: 2, 6-dimethoxy-4-(2-propen-1-yl) -phenol; Dendrobium officinale; Naringenin; Simulated moving bed; Supercritical fluid extraction
- From: Chinese Pharmaceutical Journal 2016;51(24):2155-2162
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To isolate the chemical constituents and screen out the activity index of Dendrobium officinale, and establish a reliable method for isolation and determination. METHODS: A supercritical fluid extraction (SFE) combined with simulated moving bed (SMB) chromatography method was established for the chemical composition extraction of D. officinale. RESULTS: Under the optimal SFE condition 46 g crude extract and 30 mg naringenin could be obtained from 1 kg of dried D. officinale. Naringenin and peak X could be fully extracted and isolation by the optimal supercritical fluid simulated moving bed (SF-SMB) operation. The content of naringenin increased from 1 199 mg to 2 400 mg in 1 kg of crude extract and the percentage content of peak X increased from 4.99% to 20.44%. The pure peak X was isolation by SMB and identified as 2, 6 -dimethoxy-4-(2-propen-1-yl)-phenol using GC-MS, 1H-NMR, and IR. CONCLUSION: The technology of SFE combined with SMB is stable and excellent for extraction and isolation of the active ingredients from D. officinale, and it provides a new idea for the industrial extraction.
