Determination of ropivacaine and sufentanil in maternal and neonatal plasma by UPLC-MS/MS
- Author:
Jiao WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Blood concentration; Labor analgesia; Ropivacaine; Sufentanil; UPLC-MS/MS
- From: Chinese Pharmaceutical Journal 2017;52(1):57-62
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish a UPLC-MS/MS method for the determination of ropivacaine and sufentanil plasma concentration in neonate and maternal. METHODS: Ropivacaine and sufentanil were extracted from plasma by ethyl acetate and ethyl ether (1:1, V/V), and determined by LC-MS /MS using bupivacaine as the internal standard. Separation was carried on Waters BEH C18 column (2.1 mm × 50 mm, 1.7 μm) with a mobile phase of 0.1% formic acid acetonitrile -0.1% formic acid water. The flow rate was 0.35 mL·min-1 and column temperature was maitained at 45℃. ESI source was applied and operated in positive ion mode. Quantitative determination was performed using multiple reaction monitoring (MRM) of m/z 275.2→m/z 126.0 for ropivacaine, and m/z 387.1→m/z 238.1 for sufentanil. RESULTS: There were good linearity of ropivacaine (0.25-202.0 ng·mL-1) and sufentanil (0.165-133.6 ng·mL-1), and the correlation coefficient (r2) were greater than 0.995. The extraction recovery for ropivacaine and sufentanil were more than 71.62%, while the intra-day precision (RSD) were lower than 13.86%. CONCLUSION: The method is accurate and high sensitive, providing a good analysis mean for monitoring neonate and maternal blood concentration of ropivacaine and sufentanil.
