Rapid Serological Identification for Monophasic Salmonella Serovars with a hin Gene: Specific Polymerase Chain Reaction.
- Author:
Sung Hun KIM
1
;
Shukho KIM
;
Sang Won LEE
;
Yeon Ho KANG
;
Bok Kwon LEE
Author Information
1. Division of Bacterial Enteric Infections, Center for Infectious Diseases, National Institute of Health, Seoul, Korea. bokrates@nih.go.kr
- Publication Type:Original Article
- Keywords:
Monophasic Salmonella;
hin;
PCR;
Serological identification
- MeSH:
Disease Outbreaks;
Flagella;
Korea;
Polymerase Chain Reaction*;
Salmonella Infections;
Salmonella*;
Serologic Tests
- From:Journal of Bacteriology and Virology
2005;35(4):291-298
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Salmonellosis is one of the most common food born diseases in Korea. However, it takes more than 8 days and many expensive antiserums are used for the identification of Salmonella serovars since the microorganism easily undergoes phase variation. According to the data that 65.5% of Salmonella isolates in 2000~2004 year had monophasic flagella, we have developed a rapid serological identification method using a hin gene-specific polymerase chain reaction (PCR) for monophasic Salmonella isolates that does not require the time-consuming phase conversion experiments. Using our new method, 'hin specific PCR-based serological test', we could identify serovars of monophasic Salmonella in 4 days. For the purpose of rapid identification of salmonella serovars collected from outbreaks and sporadic cases, hin specific PCR-based serological tests will be a fast and efficient method.
