Establishment of Fingerprints of Aconitum Flarum Hand. Mazz by UPLC
- Author:
Li LIN
1
Author Information
- Publication Type:Journal Article
- Keywords: Aconitum Flarum Hand. Mazz; Fingerprint; UPLC
- From: Chinese Pharmaceutical Journal 2017;52(10):865-870
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish the fingerprint of Aconitum Flarum Hand. Mazz Busch. METHODS: UPLC analysis was performed on ACQUITY UPLC BEH C18 column(2.1 mm×50 mm, 1.7 μm). Gradient elution was conducted. Mobile A was 0.2% formic acid and 0.005% TFA in water; mobile B was methanol; and mobile C was acetonitrile. The flow rate was 0.3 mL·min-1. The detection was carried out at 275 nm. The column temperature was maitained at 35℃. The injection volume was 1.0 μL. RESULTS: The UPLC fingerprint of Aconitum Flarum Hand. Mazz was established, and 14 common peaks were found including the peaks of two major constituents. The similarity factors ranged from 0.850 to 0.980. In addition, there was significant difference between wild and cultivated samples. CONCLUSION: This UPLC method has satisfactory precision, stability, repeatability and specificity. It provides a scientific method for identifying wild and cultivated Aconitum Flarum Hand. Mazz using fingerprint.
