Renal Cell Toxicity Screening of Drugs by High Content Analysis
- Author:
Zhu XIAO
1
Author Information
- Publication Type:Journal Article
- Keywords: High content analysis; Nephrotoxicity; Renal toxicity screening
- From: Chinese Pharmaceutical Journal 2017;52(14):1246-1250
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To discuss the feasibility of renal toxicity screening of drugs using high content analysis technology. METHODS: Rosiglitazone was selected as the negative drug and valinomycin was selected as the positive drug. High intension HK-2 renal cell toxicity screening model was established through testing five indexes including cell number, DNA intensity, cell permeability, mitochondrial membrane potential and cytochrome C, using high content analysis technology. Aristolochic acid A and cisplatine that has obvious renal toxicity was used to validate the model. RESULTS: Valinomycin, cisplatin and aristolochic acid A has obvious renal toxicity;rosiglitazone don't have renal toxicity. CONCLUSION: High content analysis can be used to screen renal toxicity of drugs.
