HPLC Fingerprint Analysis of Caulis of Chimonanthus nitens Combined with Chemometrics Method
- Author:
Chen-Cong ZHONG
1
Author Information
- Publication Type:Journal Article
- Keywords: Caulis of Chimonanthus nitens; Hierarchical clustering analysis; HPLC fingerprint; Principal component analysis; Quality control
- From: Chinese Pharmaceutical Journal 2017;52(21):1944-1947
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish the high performance liquid chromatography (HPLC) fingerprint of the caulis of Chimonanthus nitens and evaluate the product quality by chemometrics analysis method. METHODS: The method was developed on an Amethyst C18-H column(4.6 mm×250 mm, 5 μm)by gradient elution with acetonitrile-water (containing 0.1% formic acid) as mobile phase at a flow rate of 0.8 mLmin-1. The column temperature was maintained at 28℃, and the detection wavelength was set at 254 nm. The main characteristic peaks was identified by comparing the retention time and UV absorption characteristics. Then 10 batches of the caulis of Chimonanthus nitens were evaluated by similarity assay, HCA, and PCA. RESULTS: The HPLC fingerprint of the caulis of Chimonanthus nitens was established and three main peaks were identified. The similarity of 10 batches of the caulis of Chimonanthus nitens was about 0.978 0 to 0.991 9. CONCLUSION: The established method can be used for the quality control of the caulis of Chimonanthus nitens.
