Purification and Identification of Chemical Constituents of Pedicularis longiflora Rudolph.var. Tubiformis (Klotz). Tsoong
- Author:
Yu DENG
1
Author Information
- Publication Type:Journal Article
- Keywords: Antioxidant activity echinacoside; DPPH; Jionoside A1; Kankanoside H1; Pedicularis longiflora Rudolph.var. tubiformis (Klotz).Tsoong
- From: Chinese Pharmaceutical Journal 2017;52(24):2146-2150
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To investigate the chemical constituents of Pedicularis longiflora Rudolph. Var. tubiformis (Klotz). Tsoong systematically. METHODS: Silica-gel column, reversed phase C18 column, macroporous resin column and Sephadex LH-20 column were employed to purify the chemical constituents. The ability to remove DPPH free radicals was determined to evaluate the antioxidant activities of the crude extract, eluted fractions and compounds. RESULTS: Ten compounds were obtained and identified as (E)-3-(β-D-glucopyranosyloxy)-5-hydroxy-4'-methoxystilbene (1), β-sitosterol (2), iridotrial glucoside (3), luteolin(4), 5, 7, 4'-trihydroxy-3', 5'-dimethoxyflavone (5), echinacoside (6), jionoside A1 (7), kankanoside H1 (8), kankanoside l (9) and pedieularioside I (10) based on the NMR spectra together with physical and chemical properties. In addition, the methanol-eluted fractions and compound 6 showed moderate antioxidant activity. CONCLUSION: Compounds 1, 3 and 6-10 are isolated form Pedicularis longiflora Rudolph.var. tubiformis (Klotz).Tsoong for the first time.
