Protection Effect of Schisanhenol on Oxidative Damage Induced by H2O2 in SH-SY5Y Cells
- Author:
Yun-Feng HAN
1
Author Information
- Publication Type:Journal Article
- Keywords: Hydrogen peroxide; Schisanhenol; SH-SY5Y; Silent information regulator 1; Tau protein
- From: Chinese Pharmaceutical Journal 2018;53(4):273-277
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To investigate the protective effect and possible mechanism of schisanhenol(Sal) in SH-SY5Y cell induced by H2O2. METHODS: SH-SY5Y cells were treated with Sal (1, 10 and 50 μmol•L-1) for 4 h and then exposed to H2O2 100 μmol•L-1 for 24 h. Cell viability was measured by MTT assay. The expressions of silent information regulator 1(SIRT1), PGC-1α and p-tau (S396) protein were detected using Western blotting. RESULTS: MTT results showed that Sal significantly increased the survival rate of SH-SY5Y cell damaged by H2O2. Western blotting analysis showed that H2O2 reduced the expressions of SIRT1 and PGC-1α in SH-SY5Y cells. However, tau protein content was increased by H2O2 at p-tau(S396) sites. Sal treatment significantly increased the levels of SIRT1 and PGC-1α and decreased p-tau(S396) level induced by H2O2 in SH-SY5Y cells. CONCLUSION: These results suggest that Sal has a protective effect on H2O2 damaged SH-SY5Y cells, which is related to up regulating the expressions of SIRT1 and PGC-1α protein and decreasing the phosphorylation of tau protein.
