Resonance Rayleigh Scattering Method for Determination of Captopril in Drug and Biological Samples with Brilliant Green as Probe
- Author:
Xiang-Dong PANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Blood; Brilliant green; Captopril; Drug; Resonance Rayleigh scattering; Urine
- From: Chinese Pharmaceutical Journal 2018;53(5):383-387
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish a new resonance Rayleigh scattering method for the fast determination of captopril in drug and biological samples. METHODS: In a Tris-hydrochloric acid buffer solution of pH 9.05, quantitative methods of determination of captopril in drug and biological samples were studied by resonance Rayleigh scattering technology with brilliant green as probe. The detection wavelength was set at 368 nm. RESULTS: In the weak alkaline Tris-hydrochloric acid medium, captopril reacted with brilliant green to form a green binary ionic association complex, which led to a quench effect of the system's resonance rayleigh scattering (RRS). The maximum resonance Rayleigh scattering peak was located at 368 nm. At this wavelength, the system's RRS quenching degree was directly proportional to the mass concentration of captopril in the range of 0.005 to 0.43 mg•L-1 with the detection limit of 0.004 8 mg•L-1. The recovery and RSD (n = 6) were found to be 98.95% - 102.0% and 1.6% - 2.3%, respectively. CONCLUSION: The method is simple, rapid and sensitive, which was applied to determine the contents of captopril in commercially available captoril drugs and in human blood and urine with satisfactory results.
