Preparation of Cholesterol-PEG-Modified Amphotericin B Liposomes and the Pharmacokinetics in Rats
- Author:
Xian XIU
1
Author Information
- Publication Type:Journal Article
- Keywords: Amphotericin B; Cholesterol-PEG; Liposome; Pharmacokinetics
- From: Chinese Pharmaceutical Journal 2018;53(5):371-374
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To prepare cholesterol-PEG-modified amphotericin B liposomes and amphotericin B liposomes, and evaluate their encapsulation efficiency and pharmacokinetics. METHODS: It was employed to prepare both kinds of liposomes with the film-evaporation, the dynamic light scattering techniques and Ultrafree-CL were used to determine the mean diameter and the encapsulation efficiency, respectively. Pharmacokinetics was evaluated in Wista rats. RESULTS: Mean diameter of the liposomes was (115 ± 20) nm, and the encapsulation efficiency was over 99.9%. Moreover, the pharmacokinetic study in rats indicated that cholesterol- PEG-modified liposomes could significantly increase the ρmax and AUC. CONCLUSION: Amphotericin B liposomes prepared by film-evaporation method have high encapsulation efficiency, and cholesterol-PEG modification can prolong the circulation time of amphotericin B in vivo.
