Effects and Mechanism Research of Kangfuxin on Damp-Heat Ulcerative Colitis in Rats
- Author:
Wan-Xin YU
1
Author Information
- Publication Type:Journal Article
- Keywords: Damp-heat; Kangfuxin; Mechanism; Ulcerative colitis
- From: Chinese Pharmaceutical Journal 2018;53(11):882-887
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To study the effect of kangfuxin on damp-heat syndrome combined with TNBS-induced ulcerative colitis in rats and to explore its mechanism. METHODS: Rat model of damp-heat was established by high-fat and high-sugar diet, and then combined with TNBS to establish rat model of damp-heat, respectively, to give sulfasalazine, rehabilitation, high, medium and low dose enema, by measuring disease activity index(DAI), colonic mucosal injury index(CMDI) and histopathological score(HS). The levels of IL-8, IL-17 and IL-2, MPO, EGF and TNF-α in serum were measured by enzyme-linked immunosorbent assay(ELISA). RESULTS: Compared with the model control group, the high dose group can significantly reduce the DAI, HS and CMDI scores of the damp-heat type UC rats(P<0.01, P<0.05). Each group type can reduce the heat UC rat serum IL-8, IL-17 and expression of MPO,TNF-α in colon tissue, increases, the expression of rat IL-2 EGF(P<0.01). CONCLUSION: Kangfuxin has a certain therapeutic effect on damp-heat type UC rats, and its mechanism may be related to down-regulating the expression of IL-8, IL-17,MPO and TNF-α, up-regulating the expression of IL-2 and EGF.
