Analysis of Metabolic Components of Estrogenic Sites of Cuscuta chinensis in Serum and Feces of Rats
- Author:
Jing-Ya WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Cuscuta chinensis; Estrogenic effect; LC-MS; Metabolic pathway analysis; Metabolite
- From: Chinese Pharmaceutical Journal 2018;53(11):854-861
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To identify the metabolites of Cuscuta chinensis in the serum and feces of rats with estrogenic estrogen, and to provide the basis for elucidating the pharmacological basis of its efficacy. METHODS: Using the HPLC-ESI-MS/MS technique, the molecular weight and molecular formula of the compounds were preliminarily estimated by the first-order mass spectrometry, and the chemical constituents of serum and feces were characterized by the retention time of the standard, the fragment ion information, the fragmentation law of mass spectrometry and the reference data. RESULTS: Five prototypic components and 4 metabolites were identified in the serum. A total of 11 chemical constituents were identified in the feces after administration, including 5 prototype components and 6 metabolites. CONCLUSION: Through the comparative analysis between serum and feces, the main metabolic pathways of Cuscuta chinensis compound are deduced, which provides reference for the basic research on the estrogenic substance of Cuscuta chinensis.
