Effects of Polysaccharide from Dendrobium officinale Kimura et Migo on the Reproductive Damage in Male Mice Induced by Cisplatin
- Author:
Chun-Bin LU
1
Author Information
- Publication Type:Journal Article
- Keywords: Cisplatin; Dendrobium officinale polysaccharide; Reproductive damage; Sperm; Test
- From: Chinese Pharmaceutical Journal 2019;54(1):28-35
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To study the effects of polysaccharides from Dendrobium candidum Kimura et Migo (DOP) on reproductive damage in male mice induced by cisplatin. METHODS: Forty male Kunming mice were randomly divided into eight groups: control group, DOP low-dose group (LD-DOP), DOP medium-dose group (MD-DOP), DOP high-dose group (HD-DOP), cisplatin group, cisplatin-low-dose DOP group (cisplatin-LD-DOP), cisplatin-medium-dose DOP group (cisplatin-MD-DOP), and cisplatin-high-dose DOP group (cisplatin-HD-DOP), 5 mice in each group. The cisplatin group and cisplatin-LD/MD/HD-DOP group were intraperitoneally injected with 2.5 mg•kg-1•d-1 cisplatin for 5 d, control group and LD/MD/HD-DOP group were intraperitoneally injected with equal volume of saline. After 24 h of the last injection, the 3 DOP groups and cisplatin-DOP group were intragastrically administrated with 100 mg•kg-1•d-1 DOP (low-dose), 200 mg•kg-1•d-1 DOP (medium-dose) and 400 mg•kg-1•d-1 DOP (high-dose), control group and cisplatin group were intragastrically administrated with equal volume of saline. The mice were sacrificed after DOP treatment for 35 d, and were sampled to evaluate effects of DOP on the number of sperm, sperm quality, histopathology, as well as oxidative stress of testicular tissue in mice. RESULTS: Compared with cisplatin group, index of testis and epididymis, the number of sperm, sperm motility and viability were significantly increased (P<0.05 or P<0.01), and sperm abnormality rate was significantly decreased (P<0.01) in cisplatin-LD/MD/HD-DOP group. The SOD activity was significantly increased (P<0.01), and MDA content was significantly decreased (P<0.01) in testicular tissue. Meanwhile, histopathologicla damage of testicular tissue was significantly improved in cisplatin-LD/MD/HD-DOP group. Compared with cisplatin-LD-DOP group mice, the number of sperm was increased and sperm quality was improved, oxidative damage of the testicular tissue and histopathological damage were significantly improved in cisplatin-MD-DOP group and cisplatin-HD-DOP group mice. CONCLUSION: DOP could significantly increase sperm numbers as well as improved sperm quality, and promote the antioxidative ability of testicular tissue in cisplatin model mice, the result shows that DOP has preventing effects on reproductive damage of model mice in dose-dependent manner.
