Identification of Gentiana rhodantha and Its Adulterants by DNA Barcoding Coupled with High Resolution Melting Analysis
- Author:
Yu-Yu LIU
1
Author Information
- Publication Type:Journal Article
- Keywords: Adulterant; DNA barcoding; Ethnodrug; Gentiana rhodantha; High resolution melting; Identification
- From: Chinese Pharmaceutical Journal 2019;54(9):687-692
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To apply DNA barcoding coupled with high resolution melting analysis to distinguish Gentiana rhodantha from its adulterants. METHODS: The internal transcribed spacer 2 (ITS2) barcode was selected for HRM analysis to produce standard melting profile of the selected species. RESULTS: The ITS2 molecular regions coupled with HRM analysis can effectively differentiate five herbal species, including two Gentiana rhodantha and their four common adulterants. CONCLUSION: DNA barcoding coupled with HRM analysis is a accurate, reliable, rapid, cost-effective and robust tool, which may contribute to the quality control of traditional Chinese medicine in the natural health product industry.
