Study on Substitute Reference Substance Method with DRS Origin Software: A Case Study of Determination of 11-keto-β-boswellic Acid and 11-keto-β-acetyl-boswellic Acid in Frankincense by UHPLC
- VernacularTitle: 基于DRS origin的替代标准物质法研究——以超高效液相色谱测定乳香中11-羰基-β-乳香酸和11-羰基-β-乙酰乳香酸的含量为例
- Author:
Cai-Lin SUN
1
Author Information
- Publication Type:Journal Article
- Keywords: 11-keto-β-acetyl-boswellic acid; 11-keto-β-boswellic acid; DRS origin software; Frankincense; Relative correction factor; Substitute reference substance method; Ultra-high performance liquid chromatography
- From: Chinese Pharmaceutical Journal 2019;54(17):1411-1417
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE： To establish a UHPLC method for determination of the contents of 11-keto-β-boswellic acid(KAB) and 11-keto-β-acetyl-boswellic acid(AKBA)in Frankincense and explore the suitability and accuracy of substitute reference substance method with DRS origin software for qualitative and quantitative determination of chromatographic peaks. METHODS ：The samples were separated by UHPLC for determination of AKBA and KBA. AKBA was used as a reference to investigate the accuracy of KBA identification using DRS origin software on 19 different C18 columns. The RSDs of relative correction factors were calculated for different detection wavelengths and instruments.The relative correction factor method and the external standard method were selected for quantification and the differences were compared. RESULTS: The established method met the requirements of methodology and the average recovery was 100.21%(n=6) with RSD of 2.47%. The DRS origin software can be used to accurately determine the chromatographic peaks. The correct factor of AKBA vs. KBA was 0.936 and it was consistent under different conditions. There were no significant differences between the content calculated by the relative correction factor method and by the external standard method. CONCLUSION： This method is intelligent, feasible, reliable and economical, and can be used for the determination of frankincense content.
