Chemical Substantial Basis of Shengmai Injection(Ⅰ): Qualitative Analysis of Chemical Components
- VernacularTitle: 生脉注射液化学物质基础研究(Ⅰ)——化学成分的定性分析
- Author:
Li-Xing NIE
1
Author Information
- Publication Type:Journal Article
- Keywords: Amino acid; Lignin; Qualitative analysis; Saccharide; Saponin; Shengmai injection; Substantial basis
- From: Chinese Pharmaceutical Journal 2019;54(17):1387-1394
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE： To conduct a comprehensive and systematic qualitative investigation on chemical components in Shengmai injection. METHODS： Gas chromatography-mass spectroscopy (GC-MS) with pre-column derivatization, rapid resolution liquid chromatography-ion trap-tandem mass spectroscopy (RRLC-IT-MSn) and ion exchange chromatography (IEC) with post-column derivatization were applied in identification of saccharides, saponins, lignins and amino acids in the sample. Furthermore, preliminary investigation on test of macromolecules was performed using size exclusion chromatography-evaporative light scattering detection (SEC-ELSD). RESULTS： Fructose, glucose, sucrose, maltose, 5-hydroxymethyl-2-furaldehyde, 19 saponins, 14 lignins and 16 amino acids were found in Shengmai injection. CONCLUSION： The proposed methods are simple and accurate. The main chemical substantial basis of Shengmai injection is clarified, laying a foundation for further assay of chemical components.
