Identification of two imperatae rhizoma based on pcr-rflp
- Author:
Xiao-Yan ZHU
1
Author Information
- Publication Type:Journal Article
- Keywords: DNA barcoding; Imperatae rhizoma; PCR-PFLP; Site difference
- From: Chinese Pharmaceutical Journal 2019;54(18):1486-1490
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To identify two different characters of Imperatae Rhizoma on a molecular level. METHODS: A total of 30 samples of Imperatae Rhizoma were analyzed using DNA barcoding method based on ITS1, ITS2, psbA-trnH, matK, and rbcL genes. The DNA sequences were further used for site difference analysis. RESULTS: There is no site difference on psbA-trnH, matK, rbcL of two different characters of Imperatae Rhizoma, three key site differences on ITS1, and one key site difference on ITS2. The key site difference on ITS2 among two different characters of Imperatae Rhizoma can be recognized by the restriction enzyme HpyCH4III. CONCLUSION: ITS2 /ITS1 regions can accurately and efficiently identify two different characters of Imperatae Rhizoma, and PCR-RFLP analysis of the ITS2 region with HpyCH4III is a method to distinguish two different characters of Imperatae Rhizoma.
