Transport of Lipoamide and Lipoic Acid in Caco-2 Cell Monolayer Model
- VernacularTitle: 硫辛酰胺与硫辛酸在Caco-2细胞单层模型中的转运研究
- Author:
Xiao-Fang WU
1
Author Information
- Publication Type:Journal Article
- Keywords: Apparent permeability coefficient; Caco-2; Lipoamide; Lipoic acid; Transport
- From: Chinese Pharmaceutical Journal 2020;55(7):542-548
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To study the transport of lipoamide (LAM) and lipoic acid (LA) in Caco-2 cell monolayer model in vitro. METHODS: Effects of LAM and LA on the survival rate of Caco-2 cells were investigated by MTS, the bi-directional transport of lipoamide and lipoic acid from the intestinal cavity side (apical side, AP) to the basal side (basolateral side,BL) was investigated. The cumulative transport volume, apparent permeability coefficient (Papp) and transport percentage were calculated,and the relationships between transport volume and concentration and time were further studied. RESULTS: The transport amounts of LAM and LA were increased in time-and concentration-dependent manners, the Papps of LAM and LA (AP→BL) were 2.443 44×10-5-2.392 91×10-5 and 8.179 78×10-6-7.897 25×10-6 cm•s-1, and the Papps(BL→AP) were 2.258 13×10-5-2.214 3×10-5 and 8.267 98×10-6-7.926 73×10-6 cm•s-1, respectively. CONCLUSION: In the transport test of Caco-2 cells, LAM is superior to LA, suggesting that it is well absorbed orally and has high bioavailability. But it is still necessary to verify the pharmacokinetic data in vivo.
