Experimental Study on Enhancing the Antitussive Efficacy and Reducing the Toxicity of Dioscorea bulbifera L. by the Processing Technology
- VernacularTitle: 炮制增强黄药子止咳功效并降低其毒性的研究
- Author:
Xiao-Hui WU
1
Author Information
- Publication Type:Journal Article
- Keywords: Angelicae liquid-fried Dioscorea bulbifera L.; Attenuation; Cough relief; Hepatotoxicity; Liquorice liquid-fried Dioscorea bulbifera L.; Synergism
- From: Chinese Pharmaceutical Journal 2020;55(10):817-823
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To investigate the effects of different processing methods on antitussive and hepatotoxicity of Dioscorea bulbifera L.. METHODS The raw and processed products of Dioscorea bulbifera L., fried Dioscorea bulbifera L. (FD), wine-fried Dioscorea bulbifera L. (WD), liquorice liquid-fried Dioscorea bulbifera L. (LD) and angelicae liquid-fried Dioscorea bulbifera L. (AD) were administered to mice at a dose of 1.7 g•kg-1 for 7 d. The effects of processing on the cough relief of Dioscorea bulbifera L. were evaluated by the cough test induced by concentrated ammonia water. The detoxification effect and the preliminary mechanism of processing on Dioscorea bulbifera L. were evaluated by the detection and analysis of the relevant indicators. RESULTS: Compared with the RD group, LD and AD group had longer cough latency (P<0.05 or P<0.01) and fewer cough times (P<0.05 or P<0.01), lower levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and malondialdehyde (MDA) in liver tissue (P<0.05 or P < 0.01). ) The levels of serum AST and ALT, liver MDA, T-SOD, CAT, GSH, GPx and GST were significantly reversed (P<0.05 or P<0.01), and the levels of serum AST and ALT, liver MDA, T-SOD, CAT, GSH, GPx and GST were compared between the two groups. There was no significant difference (P>0.05). Compared with the AD group, there were more coughing times in LD group (P<0.05). Other processed products (FD or WD) had shorter cough latency (P<0.05 or P<0.01) and more cough times (P<0.05 or P<0.01) but had no effects on serum AST and ALT, liver tissue MDA, T-SOD, CAT, GSH, GPx (P>0.05) in mice induced by concentrated ammonia water. The intervention of WD only had significant reversal effect on the low GST level of liver (P<0.05 or P<0.01). The intervention of FD had no significant reversal effect on the low GST level of liver (P>0.05). CONCLUSION: Processing with Angelica sinensis and licorice could enhance the antitussive effect of Dioscorea bulbifera L. and reduce its hepatotoxicity. The mechanism of attenuation may be related to the inhibition of lipid peroxidation and the enhancement of antioxidant level in liver. The antitussive effect of two kinds of processing, stir-fried and wine-fried, is only effective, but no synergistic effect, no toxic effect and no attenuation effect are found.
