Optimization of Extraction Process of Thymol and Carvacrol in Origanum vulgare by Box-Behnken Design-Response Surface Methodology
- VernacularTitle: Box-Behnken响应面法优选牛至中百里香酚与香芹酚的提取工艺
- Author:
Jian-Wei ZENG
1
Author Information
- Publication Type:Journal Article
- Keywords: Box-Behnken design-response surface methodology; Carvacrol; Extraction process; Oregano phenol; Origanum vulgare; Thymol
- From: Chinese Pharmaceutical Journal 2020;55(11):883-888
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To optimize the extraction process of thymol and carvacrol in Origanum vulgare by Box-Behnken design-response surface methodology. METHODS: On the basis of single factor experiment, taking the sum of extraction rates of thymol and carvicol as the evaluation index, Box-Behnken design was used to investigate the effects of ethanol concentration, liquid-solid ratio and medicinal powder on the extraction rate. RESULTS: The optimal extraction parameters were as follows: ethanol concentration was 80%, liquid to solid ratio was 13∶1 (mL/g), medicinal powder passing through 40 meshes was used, and the highest extraction rate was 694.80 μg•g-1, with a small deviation from the predicted value. CONCLUSION: The optimal extraction method is simple, with low cost and good predictivity, and it can provide experimental basis for further large-scale production of thymol and carvacrol in Origanum vulgare.
