Investigation of Chemical Constituents and Sources of Zhishi Xiebai Guizhi Decoction by LC-MS/MS
- VernacularTitle: 基于液质联用的枳实薤白桂枝汤化学成分研究
- Author:
Zhen-Ni QU
1
Author Information
- Publication Type:Journal Article
- Keywords: Chromatographic peak assignment; High performance liquid chromatography-mass spectrometry; Naringin; Neohesperidin; Zhishi Xiebai Guizhi decoction
- From: Chinese Pharmaceutical Journal 2020;55(17):1421-1424
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To study the chemical constituents of Zhishi Xiebai Guizhi decoction and its single agent by high performance liquid chromatography-mass spectrometry to provide a scientific basis for the quality evaluation and control. METHODS: Agilent XDB-C18(4.6 mm×250 mm, 5 μm) column was used for HPLC analysis with acetonitrile-water as mobile phase by gradient elution. Using liquid chromatography-mass spectrometry technology to collect data in positive and negative ion mode, combined with literature reports, the main chemical components of Zhishi Xiebai Guizhi decoction were analyzed and identified. RESULTS: A total of 19 compounds were identified from Zhishi Xiebai Guizhi decoction. The main components are naringin, neohesperidin, etc. The main component types include alkaloids, steroidal saponins, glycosides, flavonoids, etc. CONCLUSION: This study comprehensively clarified the chemical composition of Zhishi Xiebai Guizhi decoction, and it has important reference value for the identification and quality evaluation of Zhishi Xiebai Guizhi decoction.
