The plasma concentration of omeprazole and metabolites 5′- hydroxy omeprazole, omeprazole sulphone are determinated by HPLC
- Author:
Liang-Qing FU
1
Author Information
1. Department.of Clinical Pharmacology, Affiliated Hospital, Academy of Military Medical Sciences
- Publication Type:Journal Article
- Keywords:
HPLC;
Omeprazole;
Plasma concentration
- From:
Chinese Pharmacological Bulletin
2002;18(3):342-344
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To set up a method which can determining the blood concentration of omeprazole and its metabolite 5′-omeprazole, omerpazole sulphone in order to study its cinical pharmacokinetics. METHODS: The blood concentration of omerpazole was determinated by HPLC. RESULTS: Calibrated standard curve of omeprazole in blood is Y = -0.004 499 + 0.001 909X (r = 0.9990), the recoveries of three concentrations 50, 500, 2 000 mg·L-1 are 90.36, 109.62, 108.91%, respectively; and the precisions are 9.86, 7.86, 15.52%, respectively. Calibrated standard curve of its metabolite 5′-OH omeprazole in plasma is Y = - 0. 003 659 + 0. 001 328X (r = 0.9970), the recoveries of three concentrations 20, 200, 1 000 mg·L-1 are 79.42%, 90.49 %, 93.04%, respectively; and the precisions are 8.95%, 4.52%, 9.73%, respectively. Calibrated standard curve of its another metabolite omeprazole sulphone in plasma is Y = 0.009 248 + 0.001 765X (r = 0. 999 2), the recoveries of three concentrations 20, 200, 1 000 mg·L-1 are 94.44%, 105.59%, 104.26%, respectively; and the precisions are 8.72, 8.58, 9.60%, respectively. After 20 mg omeprazole were administered by a voluteer via oral, Cmax of 5′-OH omeprazole, omeprazole, and omerpazole sulphone were 14.622 7, 408.433 2, 454.363 7 mg·L-1. CONCLUSION: The method is good enough to study pharmacokinetics of omeprazole.
