Effects of endothelin-1 on the expression of ClC-3 chloride channel in bovine cerebrovascular smooth muscle cells
- Author:
Lin-Xi CHEN
1
Author Information
1. Department of Pharmacology, Sun Yat-sen Univ. of Med. Sciences
- Publication Type:Journal Article
- Keywords:
Cerebrovascular;
Chloride channel;
ClC-3;
Endothelin-1;
Smooth muscle cell
- From:
Chinese Pharmacological Bulletin
2002;18(3):260-264
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of endothelin-1 (ET-1) on ClC-3 chloride channel protein expression in cultured bovine cerebrovascular smooth muscle cells (CSMC). METHODS: Cell culture and Western blot. RESULTS: 1 The endogenous ClC-3 expression was found in basilar artery, middle cerebral artery, and microvessel; 2 The molecular weight of expressed ClC-3 chloride channel protein was about 95 ku; 3 ET-1 enhanced ClC-3 protein expression which was inhibited by nifedipine and SK&F96365. Cyclopiazonic acid(CPA) increased the expression of ClC-3 protein in a concentration-dependant manner, and enhanced ET-1 effect on this protein expression. CONCLUSION: ET-1 stimulated ClC-3 chloride channel protein expression in cultured bovine cerebrovascular smooth muscle cells. The intracellular Ca2+ plays an important role on signal transduction pathway in ClC-3 protein expression process.
