Apoptosis regulated by (-)-epigallocatechin-3-gallate in nasopharyngeal carcinoma cells using apoptosis cDNA expression array
- Author:
Yan ZHAO
1
Author Information
1. Cancer Research Institute, Xiangya School of Medicine, Central South University
- Publication Type:Journal Article
- Keywords:
Apoptosis;
cDNA expression array;
EGCG;
LMP1
- From:
Chinese Pharmacological Bulletin
2002;18(3):255-259
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the mechanism of apoptosis induced by (-)-epigallocatechin-3-gallate (EGCG) and the intervenient effect of EGCG on apoptosis inducted by Epstein-Barr (EB) virus latent membrane protein 1 (LMP1). METHODS: The established doxycycline regulated nasopharyngeal carcinoma cell line pTet-on-LMP1 HNE2 were exposed to 0.6 mg·L-1 Dox and 100 mg·L-1 EGCG for 24 h. Total RNAs were extracted from cells hybridized with the Atlas apoptosis cDNA expression array membrane containing mainly apoptosis related genes. RESULT: EGCG regulated several apoptosis related genes, they were either up- or down-regulated. CONCLUSION: EGCG induced apoptosis might be mediated through some specific genes and signal transduction pathways, and the presence U of EGCG showed some intervenient effect on LMP1 induced cell apoptosis.
