Studies of cytotoxin from guangxi cobra venom on its purification and cytotoxic effects on the nasopharyngeal and other cancer cells
- Author:
Dan-Qing LEI
1
Author Information
1. Institute of Snake Venom Research, Guangri Medical University
- Publication Type:Journal Article
- Keywords:
Cytotoxicin;
MTT;
Tumor cell line
- From:
Chinese Pharmacological Bulletin
2002;18(1):61-64
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the inhibitory effect of the cytotoxin (CTX) from guangxi cobra venom on human nasopharyngeal cancer cell (CNE) and other tumons cells. METHODS: The cytotoxin was isolated and purified from Guangxi cobra venom by successive chromatography on Sephadex G-50 and CM Sepharose CL-6B columns. Its cytotoxic effects and does-effect relationship on human tumors cell lines were examined by MTT assay. RESULTS: The inhibitory effects of CTX CM-5 on CNE, human ovarian carcinoma cell (Ho8990), uterus cervical carcinoma cell (HELA) and lymphoma (YAC) cell lines showed a definite does-effect relationship. The IC50 (48 h in cubation) was 1.84, 2.59, 1.84 and 0.75 mg·L-1 respctively. The inhibitory effects of CTX CM-5 on CNE increased with time. The IC50 (3 h and 24 h cubation) was 4.78 and 1.04 mg·L-1 respctively. CONCLUSION: CTX from Guangxi cobra venom exhibites strong suppressive effect on cultured tumor cells line in vitro.
