Neuroprotective effect of salidroside on CoCl2 - Induced hypoxia injury in PC12 cells
10.3969/j.issn.1001-1978.2019.04.017
- Author:
Ze-Lin YANG
1
Author Information
1. College of Pharmacy, Fujian University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Complement component;
Egr4;
Hypoxia injury;
Neuroprotection;
PC12;
Salidroside
- From:
Chinese Pharmacological Bulletin
2019;35(4):530-534
- CountryChina
- Language:Chinese
-
Abstract:
Aim: To investigate the neuroprotective effect of salidroside on CoCl2 -induced hypoxia injury in PC 12 cells, and further explore the mechanism of salidroside on inhibiting complement and neuroprotection. Methods: The model of CoCl2-induced hypoxia injury in PC 12 cells was established. The expressions of Egrl, Egr4, C3 and the activity of caspase-3 were assessed with different concentrations of salidroside treatment. The expressions of Egrl, Egr4 and the activity of caspase-3 were tested with C3ar inhibitor and salidroside treatment. The expressions of Bax, Bcl-xl, C3 and the activity of caspase-3 were determined with Egr4 siRNA and salidroside treatment. Results: Salidroside significantly reduced the caspase-3 activity and the protein expression of Bax and C3, and improved the expression of Bcl-xl, Egrl, and Egr4 proteins in CoCl2-induced PC12 cells. Salidroside reduced the caspase-3 activity and the expression of Egrl, Egr4 with C3ar inhibitor treatment. With siRNA interference with the expression of Egr4, salidroside reversed the activity of caspase-3, Bax and Bcl-xl, but the effect on C3 was not obvious. Conclusions: Salidroside has neuroprotective effect on the model of CoCl2 -induced hypoxia injury in PC12 cells, which is related to the inhibition of complement component C3 and the improvement of the expression of Egr4 by salidroside.
