Ameliorative effect of salvianolic acid b on renal fibrosis in diabetic rats and potential mechanisms implicated
10.3969/j.issn.1001-1978.2019.01.012
- Author:
Xu ZHANG
1
Author Information
1. Dept of Pharmacology
- Publication Type:Journal Article
- Keywords:
Diabetes;
Diabetic nephropathy;
Oxidative stress;
Renal fibrosis;
Salvianolic acid b;
Tgf-pi
- From:
Chinese Pharmacological Bulletin
2019;35(1):51-55
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the ameliorative effect of salvianolic acid B (Sal B) on renal fibrosis in dia-betic rats and the possible mechanisms implicated. Methods Diabetes in SD rats was induced by high sugar high fat diet combined with intraperitoneal injection of streptozotocin (STZ, 40 mg • kg"1). Diabetic rals were allocated randomly to model group, high dose of Sal B group (160 mg • kg"1) and low dose of Sal B group (80 mg • kg"1). Rats in Sal B groups were administered intragastrically with corresponding dose of Sal B for six weeks, while those in the control and model group were given distilled water of the same volume. At the end of experiment, the levels of fasting blood glucose (FBG) , serum creatinine (SCr) , blood urea nitrogen (BUN), urine microalbumin (UAlb), serum malondialdehyde (MDA), total antioxidant capacity (TAC) and superoxide dismulase (SOD) were determined with commercially available kits. Renal fibrosis was evaluated by Masson staining and renal contents of type I and type III collagen determined by ELJSA. The protein levels of TCF-pl, p-Smad2 and Smad7 were evaluated by Western blot analysis. Results In model group, the levels of FBG, SCr, BUN, UAlb and MDA significantly increased as compared with those in control group, while TAC and SOD activities decreased markedly ( P < 0. 01 ). Bats in model group exhibited evident renal fibrosis, accompanied by significantly increased levels of TGF-f}l and p-Smad2 protein and decreased level of Smad7 protein in renal tissues (P <0. 01). Supplementation with Sal B significantly ameliorated renal fibrosis in diabetic rats, with TGF-pl and p-Smad2 protein down-regulated and Smad7 protein up-regulated evidently ( P < 0. 05 or P <0.01). The above mentioned changes in biochemical parameters were also reversed markedly in Sal B treated groups (P < 0.05 or P < 0. 01). Conclusions Sal B is capable of ameliorating renal fibrosis in diabetic rats, which might be related to its antioxidant property and regulation of TGF-pl/Smad signaling pathway.
