Apoptosis of gastric cancer MGC-803 cells induced by rosmarinic acid analogue-11 by inhibiting ERK/MAPK pathway
10.3969/j.issn.1001-1978.2019.02.013
- Author:
Wan-Ting LI
1
Author Information
1. Dept of Pharmacy, First Affiliated Hospital, Guangxi Medical University
- Publication Type:Journal Article
- Keywords:
Apoptosis;
ERK/MAPK;
Gastric cancer;
MGC-803;
Proliferation;
RAA-11
- From:
Chinese Pharmacological Bulletin
2019;35(2):208-213
- CountryChina
- Language:Chinese
-
Abstract:
Aim: To explore the apoptosis and mechanisms of human gastric cancer cells MGC-803 induced by RAA-11. Methods: MTT and colony assays were used to detect the survival of MGC-803 cells treated with RAA-11. The effect of RAA-11 on the apoptotic morphology of MGC-803 cells was observed by Hoechst 33258 staining. The effect of RAA-11 on the apoptosis rate of MGC-803 cells was detected with flow cytometry. The effects of RAA-11 on apoptosis related protein expression of caspase-3, Bcl-2 and Bax as well as pathway proteins ERK and p-ERK in MGC-803 cells were observed by Western blot. Results: MTT assay showed that the proliferation of MGC-803 cells was effectively inhibited in a time- and concentration-dependent manner (P <0. 01). The result of colony suggested that RAA-11 could inhibit the proliferation of MGC-803 cells. Hoechst 33258 staining indicated that the nucleus of MGC-803 cells had a typical apoptotic morphological change after intervention with RAA-11. The result of flow cytometry suggested that RAA-11 had a significant apoptotic effect on MGC-803 cells. The expressions of Bax and caspase-3 were significantly up-regulated (P <0. 01), and the expressions of Bcl-2, ERK and p-ERK were down-regulated (P <0. 01) in MGC- 803 cells treated with RAA-11. Conclusions: RAA-11 inhibits the proliferation and induces apoptosis in MGC-803 cells, which may be related to the inhibition of ERK/MAPK pathway.
